18.97.14.82
18.97.14.82
close menu
SCIE
Bacillus sp . CW - 1121 이 생성하는 단백 분해 효소를 이용한 참깨박 단백질의 용출
Extraction of protein from defatted sesame meal using the enzyme from Bacillus sp . CW -1121
최청(C . Choi), 천성숙(S . S . Chun), 조영제(Y . J . Cho)
UCI I410-ECN-0102-2008-520-000754111

To extract insoluble proteins of sesame meal residue by using microorganism, the sesame meal residue was treated with crude enzyme solution from Bacillus sp. CW-1121. It was found that the solubility reached to maximum at pH 7.5, 45℃. Under optimum condition, the nitrogen solubility with the enzyme solution from Bacillus sp. CW-1121 reached to 60% in 2 hours. Nitrogen solubility of protein from sesame meal showed minimum value at pH 4.5 and significantly increased above pH 6.0. When the protein from sesame meal extracted with Bacillus sp. CW-1121 was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis, water soluble protein was showed 4 bands and salt soluble protein was showed 2 bands. The amino acid composition of water soluble protein, salt soluble protein and free amino acid indicated relatively high contents of serine (17.24 ㎎/g), glutamic acid (10.77 ㎎/g) and glutamic acid (6.55 ㎎/g). Specially, the contents of essential amino acids were high.

[자료제공 : 네이버학술정보]
×