3 Tocotrienol-α-, γ- and δ-tocotrienol-from latex(Hevea Brasiliensis) were isolated and the oily tocotrienol concentrates obtained. To isolate tocotrienols, the fractionation by semipreparative HPLC of the unsaponifiable fraction in the raw lipid extract from latex was carried out. By this method, the total content of tocotrienols in latex was ca. 400 ppm, and the purities of α-, γ- and δ-tocotrienol were 98.3, 99.3 and 96.2%, respectively.