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SCIE
새로운 반응기구에 의한 bradykinin 유사물의 합성
Synethesis of bradykinin analogues by new reaction vessel
최청 ( Cheong Choi )
UCI I410-ECN-0102-2008-520-000740993

Synthesis of (D-Phe^7 -Leu^8) bradykinin and bradykinin by solid phase method using a new reaction vessel was carried out. Coupling was performed by dicyclohexylcarbodiimide. After cleavage with dried HBr the peptides were purified by high pressure liquied chromatography. Their purify was assayed by paper and thin layer chromatography, melting point and amino acid analysis. (D-Phe^7-Leu^8) bradykinin and bradykinin were incubater in vitro endopeptidase (α-chymotrysis) and exopeptidase(carboxypeptidase A, leucine aminopeptidase) in order to study the degradation pattern of peptides. (D-Phe^7-Leu^8) bradykinin and bradykinin were rapidly degradated by α-chymotrypsin and carboxypeptidase A (D-Phe^7-Leu^8) bradykinin and bradykinin coution(D-Phe^7 -Leu^8) bradykinin and bradykinin contain imino peptide bound from proline at N-terminal and therefore they were not attacted by leucine aminopeptidase.

[자료제공 : 네이버학술정보]
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