When cultured soybean immature seed on 15th days after flowering, suitable temperature in formation of callus were 24∼27℃, and embryogenic callus(EC) were generated at medium containing NAA with growth regulators, and then,. results were subcultured the EC, a plenty of shoots and roots were formed at medium supplemented BA 2㎎/1 and IAA 2㎎/1, respectively, however when used at medium the same time supplemented BA 2㎎/1 and IAA 2㎎/1, formation of cullus was energetic, and a symptom of organization was not showed. Total lipid contents include in each cultures were increased at low temperature of cultural conditions as much as possible, but glycolipid, phospholipid, free sterol contents were a little increased at 24∼27℃, and free sterol content was increased at a case of embryogenic structure were generated. In fatty acid compositions in each cultures, the contents of unsaturated fatty acid were plenty in EC, and unsaturation rate was 0.837. Besides, in sterol composi lions, cholesterol content was remarkably high in EC than that of other cultures.