Ascorbate Oxidase 를 이용한 Ascorbic Acid 의 분광분석법에 의한 정량

박근형; 김정현; 현규환; 김동연

한국응용생명화학회 한국응용생명화학회지 Ascorbate Oxidase 를 이용한 Ascorbic Acid 의 분광분석법에 의한 정량

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Ascorbate Oxidase 를 이용한 Ascorbic Acid 의 분광분석법에 의한 정량

Spectrophotometric Assay of L - Ascorbic Acid with Ascorbate Oxidase

박근형(Keun Hyung Park), 김정현(Jung Hyun Kim), 현규환(Kyu Hawn Hyun), 김동연(Dong Youn Kim)

한국응용생명화학회 1988.01

한국응용생명화학회지 vol. 31 iss. 1 52-57(6pages)

UCI I410-ECN-0102-2008-520-000750653

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Abstract

The difference of absorbance before and after oxidation of L-ascorbic acid(AsA) from cucumber was directly proportional to the concentration of AsA. The effect of pH, enzyme concentration and reaction time on the AsA assay were examined. The values of AsA obtained by the present method and HPLC method agreed well in all cases.

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