Among the Basidiomycetes, Pleurotus ostreatus 301 and Lentinus edodes 3-1 were chosen because of their good enzyme productivity and rapid mycelial growth in rice straw medium, Their cultural conditions adequate for the enzymes production and effects of various materials and inorganic salts added to the rice straw media were investigated. L. edodes 3-1 was excellent in productivity of cellulase and xylanase, and P. ostreatus 301 in protease. The optimum condition s far enzyme production were at 30℃ in cellulase production and at 25℃ in xylanase and protease production, with 75% moisture content and 5.0-6.0 initial pH. The appropriate cultural periods, for enzyme production were 30 days and 35 days for P. ostreatus 301 and L. edodes 3-1, respectively. Among the various materials added, defatted soybean. defatted rape seed, or defatted sesame were all effective to enzyme production but reduced mycelial growth. Rice bran was also effective, particularly at 30% concentration. The addition n of inorganic salts showed effective to enzyme production. Among inorganic salts. optimum concentration of CaCO₃ was 5% and that of CaSO₄was 2%.