To the rabbit and guinea pig, tartrazine was administered, either parenterally through venous or peritoneal injection or orally, with the amount of 7.5-50 ㎎/㎏ body weight in case of rabbit and 1,000-2,000㎎ per animal in case of guinea pig, in order to observe acute toxic effect of the dye in the animals. Blood serum was analyzed chemically and all the internal organs were microscopically observed for this purpose. The results point to the fact that severe hemorrhagic and congestive findings in most of the organs can be induced by the amount of the dye utilized, and particularly liver damage could be resulted.