18.97.14.83
18.97.14.83
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SCIE
Cellulase 에 관한 연구 - ( 제1보 ) Cellulase 생성균의 분리와 조효소의 제성질 -
Studies on Cellulase - Part 1 . Isolation of Cellulase Forming Microorganisms and the Properties of Crude Enzymes -
정동효 ( Dong Hyo Chung )
UCI I410-ECN-0102-2008-520-000756413

Out of some 400 strains of Microorganisms, cellulase forming organisms was isolated from night soil during the course of screening tests. Two strains, Ku-3371 and Ku-4383 were found capable of producing cellulase in the shaking culture. General properties of the crude enzyme were as the following results. 1. The optimum pH values on CMC-saccharifying, CMC-liquefying and filter paper disintegrating activities were 4.0 to 5.5. 2. The stable pH range was within 3.5 to 6.5, 3. The optimum temperature was 40-45℃, the thermal stability was below 50℃ except on paper disintegrating activity and completely inactivated at 70℃. 4. Dialyzed crude enzyme was activated by Mn^(2+) and Co^(2+) respectively but Hg^(2+) was strong inhibitor.

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