Effects of Bupleuri Radix, Paeoniae Radix or Uncariae Ramulus et Uncus on experimental hyperlipidemia were studied. Hyperlipidemia was induced on male Wistar rats by feeding them on high cholesterol diet for one week, as previously described by the authors. Blood lipid profile was varified on these rats by measuring total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL) and low density lipoprotein cholesterol(LDL). Then, the rats were followed by feeding normal diet. At the same time, methanol extracts of the three individual herb medicines were orally administered to the rats for 4 weeks and the parameters above mentioned were monitered. Methanol extract of Bupleuri Radix reduced the TC value significantly at 2 week point and Paeoniae Radix reduced the TC value at 4 week point in compare to control group, suggesting the antihyperlipidemic effect of the two herbal medicines in vivo. The extract of Uncariae Ramulus et Uncus however did not show antihyperlipidemic effect in our experiment.