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청청벼에서 유래한 벼멸구 저항성관련 RAPD Marker의 개발
Development of RAPD Marker Related to Brown Planthopper Resistance Gene Derived from Rice Cultivar, Cheongcheongbyeo
서지훈 ( Ji-hun Seo ) , 김경민 ( Kyung-min Kim ) , 김석만 ( Suk-man Kim ) , 손재근 ( Jea-keun Sohn )
UCI I410-151-25-02-091958976
이 자료는 4페이지 이하의 자료입니다.

This study was carried out to select DNA markers closely linked to brown planthopper (BPH) resistance gene originated from a rice cultivar ‘Cheongcheongbyeo’. For the mapping of resistant gene to BPH, a doubled-haploid (DH) population was developed by anther culture of F1 plants from a cross ‘Cheongcheongbyeo/Nagdongbyeo’. In BPH bioassay and marker screening for the DH population, the segregation of resistant and susceptible plants to HPH fitted to a 1:1 ratio. A total of 310 RAPDs of 520 markers showed polymorphism in parental survey using ‘Cheongcheongbyeo’ and ‘Nagdongbyeo’. In the analysis of relationship between BPH resistance and marker pattern for 40 DH lines, the OPE16 produced a specific dominant fragment, 700 bp, which was closely linked with BPH resistance gene of ‘Cheongcheongbyeo’. Based on the linkage analysis using 7 markers, BPH resistance of ‘Cheongcheongbyeo’ was mapped on chromosome 12, which was closely linked with OPE16700 at a distance of 4.6 cM.

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