Anthocyanin contents of black soybean were analyzed for development of superior breeding lines with high anthocyanin contents. Total 292 genotypes of black soybean collected through the whole country were analyzed by HPLC in which C3G (Cyanidin-3-Glucoside), D3G (Delphinidin-3-glucoside), and Pt3G (Petunidin-3-Glucoside) were detected main anthocyanin pigments and each pigment contents were significantly different according to genotypes. C3G content showed the highest value in all materials and its variation was also wide, whereas D3G and Pt3G were not detected in 4 and 24 genotypes. Mean value of C3G, D3G, and Pt3G contents were 8.05±4.225, 1.80±0.854, and 1.15±0.781 mg/g, respectively. In case collected sites, genotypes collected in Chungnam region were higher the anthocyanin contents than other collections, which was 13.75±3.861 mg/g. It might be concluded that it takes more than 36 days for anthocyanin accumulation since beginning of seed-coat pigments formation, in that case it showed 13.09± 4.190 mg/g. Also total anthocyanin contents were present higher concentration in seed coat as maturation period was longer from flowering stage.