The salt stress at seedling stage of barley was examined in different concentrations of NaCl containing 1/2 Hoagland solution. Among the physiological factors electric conductivity and sodium content of cell sap outflow solution were increased according to the increase of NaCl concentration in 1/2 Hoagland solution but the total protein content of root cell sap outflow solution was decreased. Changing pattern of amino acids content in plant was divided into 3 groups which were increased, decreased or unchanged by NaCl concentration in solution. In normal condition, sugar content in plant was higher than potassium or sodium but if NaCl was added more than 25mM in 1/2 Hoagland solution, sodium was the highest. Potassium content in shoot was decreased as 67% of control at 100mM of NaCl containing solution and in root it was decreased as 8%. Sum of the potassium and sodium content in shoot was seriously increased in accordant with NaCl concentration in culture solution, but in root it was gradually decreased. Chlorophyll content per g fresh weight and photosynthetic ability per cm² in first leaf were not affected by NaCl concentration of 1/2 Hoagland solution but root activity was slightly decreased compared to control.