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옥수수 自殖系統들에서 化學的 突然變異 誘發物質 처리에 따른 apomixis 誘發과 胚珠發生
Induction of Apomixis by Chemical Mutagen Treatment and Ovule Development in Inbreed lines of Corn
李浩鎭 ( Ho Jin Lee ) , 崔根鎭 ( Keun Jin Chai ) , 金泰勳 ( Tae Hoon Kim )
UCI I410-151-25-02-091946216

Ml plants which were produced from seed soaking in chemical mutagen, EMS or NaN3, appeared wide morphorogical variations such as dwarf, albino, twisted leaf, white streaked leaf, and purpled stem. In mutants of reproductive organs, there were monoecious plants such as female-flower plant and male-flower plant, multiple spikes, and steriled plants among Ml plants. Also, barren stalk was increased significantly in Ml plants. Ear bagging at ear initiation stage prevented seed set on cob in normal plants. In spite of ear bagging, Ml plants which had cobs with seed set was 3.9-11.2% of stalks developed from seeds soaking with mutagens, but only three or four kernels could be matured on a cob. Ear bagging after mutagen injection into initiating ear produced 5.1-10% in cobs with seed set, but only 1.7-6.3 kernels could be matured. Cobs removed silk at four hours after artificial pollination increased the rate of cobs with seed set to 27%. Microscopic observation confirmed that ontogeny of kernels matured from ear bagging and mutagen treatment would be both adventitious and diplosporous apomictic reproduction. Chromosome set of M2 seedling was found to be diploid type in chromosomal counting of root tip. As M2 plants showed an uniform appearence within each lines and their CV of plant height were ranged 4-6% in each lines, we concluded that they were apomictic progeny. But we could not find any marker traits combined with apomixis.

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