Purpose: This study was conducted to describe the visual differentiation ability for pressure ulcer (PU), knowledge of the pressure injury classification system, attitudes, and ability to perform PU assessment and preventive nursing performance, and to examine the relationship between these variables. Methods: This was a descriptive, cross-sectional study. Data were collected from 154 new nurses working at tertiary hospitals in B metropolitan city. Results: Both visual differentiation ability for pressure ulcer and the performance of PU assessment and preventive nursing performance were positively correlated with knowledge of the pressure injury classification system and attitudes toward pressure ulcer prevention. Conclusion: The results of this study suggest that developing visual educational content, such as photographs and case studies, may improve visual differentiation ability for pressure ulcers. Additionally, the development of guidelines for implementing evidence-based nursing is recommended.