To better understand the emic perspectives of Korean organizational working society, the Korean Burnout Syndrome Inventory (KBSI) was developed. The purpose of this study was to evaluate the validity of physiological indicators with the results obtained from the KBSI through the secretion of hormones in the blood. First, all participants were divided into three groups (i.e. burnout diagnosis group, potential risk group, and general) according to the response scores on the KBSI. Second, differences in hormone levels (Cortisol, DHEA-s, and cortisol/DHEA-s) in the blood related to burnout among three groups were collected and analyzed. The cortisol concentration in the burnout diagnosis group was statistically significantly higher than those in the potential risk group and the general group. In terms of DHEA-s, the burnout diagnosis group showed the highest score but presented no significant difference statistically against the potential risk group. In the comparison of the ratio of cortisol to DHEA-s (cortisol/DHEA-s ratio), the burnout diagnosis group showed statistically significantly lower than the other groups. In sum, sufficient clinical validity has been secured to measure the status of individuals’ burnout levels through the KBSI, without additional physiological analysis to check the status of individuals’ burnout levels.