Despite progress in agricultural prosperity, agriculture faces some problems such as the drop in profitability and the reduction in deforestation, necessitating development of an effective and productive agricultural system. To encourage a productive agricultural system, biosystems engineering have a important role in the impending agri-tech revolution to promote the proper regulatory arrangement. Recently, nanotechnology regards as emerged technology to support biosystems engineering for sustainable agriculture incorporating food security and human health. In this study, we proposed nano-enabled biosystems engineering for future advanced agriculture. Specifically, we reveal that nanomaterials (i.e., graphene) can promote the plant and crop growth, showing the enhanced florescence and the increased sugar content. In addition, biocompatible nanoparticles can improve the pesticide performance and plant growth without cytotoxicity. In addition, nanotopography inspired by extracellular matrix can induce the animal tissue regeneration. Using nano-enabled biosystems engineering, we can confirm their potential use for improving functions of biosystems in the near future.