Atopic dermatitis (AD) is the most common inflammatory skin disease, characterized by abnormal skin barrier function and type 2 inflammation. It is well known that stratum corneum (SC) lipids, including ceramides, play pivotal roles in the basis of the skin barrier. On the other hand, little is known about the circulating lipids in patients with AD, although circulating lipids work as biomarkers in several other disorders. In this lecture, I talk about (i) the possibility that SC ceramides could be biomarkers, indicating complete remission or potential flares in patients with AD, and (ii) the multiple circulating lipids in patients with AD are altered, and some of them are associated with the clinical status of AD. Until now, only SC lipids have been focused on AD. Evaluating SC lipids and circulating lipids in AD provides new insight into AD's pathogenesis and therapeutic strategies.