Objective: The purpose of this study was to investigate the effects of three rotational jump conditions (standing jump, left rotational jump and right rotational jump) on stability through center of pressure (COP) and EMG variables analysis. Method: A total of 16 college students (age: 24.13 ± 7.17 years, height: 169.24 ± 8.23 cm, weight: 65.65 ± 13.88 kg) participated in this study. The study used wireless two COP plates and wireless eight channel EMG. The analyized variables were 11 variables for COP and RMS for EMG, which were analyzed using one-way analysis of variance with repeated measures according to three rotational jump conditions. Results: Among the COP variables, left rotational jump (LRJ) and right rotational jump (RRJ) were larger than standing jump (SJ) for left and right amplitude, area, total displacement, and average velocity for both feet among the variables of COP, and for area of the left foot, RRJ was larger than that of SJ. Among the EMG variables, there was no statistical difference between the muscle activations, but the muscle activity was significantly higher in the order of RRJ, LRJ, and SJ according to direction of rotation. Conclusion: Although the results of COP and EMG were not consistent through this study, it can be expected that the differences in COP was due to the amount of rotation during rotational jump-landing in the left and right directions, and that the EMG is determined by the lateral movements required for rotation.