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KCI 등재
유전자 분석 기반 수입산 형태 변이 반하 유통 사례 보고
A Case Report of Imports Morphological Variation of Pinelliae Tuber Based on the Genetic Analysis
김욱진 ( Wook Jin Kim ) , 최고야 ( Goya Choi ) , 노수민 ( Sumin Noh ) , 문병철 ( Byeong Cheol Moon )
UCI I410-ECN-0102-2023-500-000972977

Objectives : The purpose of this study is to report that applying the genetic discrimination method to Pinelliae Tuber is suitable as a countermeasure for the limitations of morphological identification announced publicly in the Ministry of Food and Drug Safety(MFDS). Methods : Randomly selected fifty samples in Pinelliae Tuber imported from China were used for morphological and genetic identification. The morphological identification was applied method announced publicly by the MFDS. The traits of morphological identification were classified as Pinellia ternata, P. tripartita, Pinellia pedatisecta, and Typhonium flagelliforme, according to the formation of tuberous root and tuber morphology. The genetic identifications were conducted by Sequence Characterized Amplified Region(SCAR) marker and DNA barcoding analysis for cross-validation, respectively. SCAR marker was verified according to the presence or absence of amplicon through PCR amplification using species-specific primers. DNA barcoding analysis used sequence information of the mat K region. Results : As a result of the morphological identification, 27 out of 50 samples were identified as original species ‘P. ternata’ of genuine ‘Pinelliae Tuber’, and 23 were identified as adulterant species ‘P. pedatisecta’. Unlike this, the genetic identification was identified as the original species ‘P. ternata’ in all 50 samples in the SCAR marker and mat K regional sequence analysis. Conclusions : Pinelliae Tuber of morphological mutant that can not be classified by morphological identification is imported from China. The SCAR marker would be used as accurate and efficient assays for species identification of the morphological mutant.

Ⅰ. 서 론
Ⅱ. 재료 및 방법
Ⅲ. 결 과
IV. 고 찰
Ⅴ. 결 론
감사의 글
References
[자료제공 : 네이버학술정보]
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