PURPOSE: This study aimed to investigate the effect of dynamic warm-up (DW), including DW and self-myofascial release (SMFR), on soft tissue stiffness and exercise performance. METHODS: Eight adult men participated in this study. The procedure was conducted twice every 2 weeks. Soft tissue stiffness measurements and exercise performance were performed as a pre-post-test. DW only and DW with SMFR were performed as an exercise intervention for the two groups, respectively. RESULTS: In the group conducted solely by DW, the stiffness of the gastrocnemius and rectus femoris muscles increased (p<.05), and in the DW group including SMFR, the stiffness of the rectus femoris muscle (p<.01) and biceps femoris muscle increased (p<.05). The Achilles and patellar tendon showed no significant changes in either group. A significant increase was observed in both the DW group and the DW group containing SMFR in the 30 m sprint (p<.01). The vertical jump (VJ) showed no significant change in both groups. CONCLUSIONS: Therefore, DW, including DW with SMFR, increased muscle stiffness, specifically through a 30-m sprint. In a follow-up study, it is considered necessary to investigate the effect of the VJ through the development of a DW program that changes the stiffness of tendons.