The amphibian breeding habitat is confirmed by mating call. In some cases, the researcher directly identifies the amphibian individual, but in order to designate the habitat, it is necessary to map the mating call region of the amphibian population. Until now, it has been a popular methodology for researchers to hear mating calls and outline their breeding habitats. To improve this subjective methodology, we developed a technique for mapping mating call regions using Unmanned Aerial Vehicle (UAV). The technology uses a UAV, fitted with a sound recorder to record ground mating calls as it flies over an amphibian habitat. The core technology is to synchronize the recorded sound pressure with the flight log of the UAV and predict the sound pressure in a two-dimensional plane with probability density. For a demonstration study of this technology, artificial mating call was generated by a potable speaker on the ground and recorded by a UAV. Then, the recorded sound data was processed with an algorithm developed by us to map mating calls. As a result of the study, the correlation coefficient between the artificial mating call on the ground and the mating call map measured by the UAV was R=0.77. This correlation coefficient proves that our UAV recording system is sufficiently capable of detecting amphibian mating call regions.