Objectives: This study aimed to analyze the correlation among dental hygiene students’ life stress, grit, and career decision-making self-efficacy, and to identify the factors influencing career decision-making self-efficacy. Methods: Data were collected through a survey from October 19 to November 7, 2020, targeting 196 students enrolled in dental hygiene departments in Daejeon and Daegu. Life stress, grit, and career decision-making self-efficacy were measured. For data analysis, the descriptive statistics, t-test, ANOVA, Pearson’s correlation coefficient, and stepwise multiple regression analysis were performed using SPSS/WIN 24.0. Results: Life stress was 2.12 points, grit was 3.07 points, and career decision-making self-efficacy was 3.43 points. Career decision-making self-efficacy was influenced by satisfaction with the course department, as well as life stress and grit; the most influential factor was found to be grit. These variables could explain career decision-making self-efficacy by 23.8%. Conclusions: For students to advance successfully into society as dental hygienists, a career decision-making self-efficacy promotion program that can help students achieve self-confidence and establish career goals should be developed. additionally, a program that can enhance grit should be included in this development.