Objective: Self-monitoring of blood glucose (SMBG) is important for patients to measure blood glucose levels and understand the factors influencing blood glucose. By increasing the motivation for SMBG, patients gain high-quality blood glucose control. Various lancing devices have been developed, and improvements such as downsizing, puncture accuracy, lower pain, and safety were reported. Naturalet Petit (NP) is less painful than the conventional lancing device. We investigated the evaluation of the SMBG questionnaire in the clinic. Methods: We enrolled 59 patients who were over 18 years old and were using SMBG. We administered the questionnaire twice, using Pocket Lancet [PL] and NP, at 1-month intervals. The patients chose which fingers to lance, and the finger site was not specified. The SMBG questionnaire included seven important points: easy to use, lower pain, easy to hold, safety, size, design, lower mistakes, portability, disposable, and 10 steps evaluation. Results: Patients have sought an easy-to-operate (69.5%) and painless (66.1%) finger-prick device. Less fear [7.59 to 8.98 (P<0.0001)] and pain [6.53 to 8.49 (P<0.0001)] were experienced after shifting from the pocket lance to the NP. However, many patients reported its difficulty to operate [easy to use: 7.34 to 6.08 (P=0.011)]. The lack of pain was considered in the decision to continue using NP [pain (OR=1.776, P=0.005, 95% CI 1.194, 2.642)]. Conclusion: A painless SMBG is ideal, but while a shallower needle depth reduces fear, it also draws blood less efficiently. It is also difficult to adjust the needle depth using disposable finger-prick devices. Different puncture methods to reduce pain may be established. Treatment strategies and devices should be selected in consideration to the patient and not based on the medical staff's convenience. Thus, the medical staff is recommended to align themselves with the patient and allow them to choose which devices to use.