Given the global demand for green energy, the battery industry is positioned to be an important future technology. Lithium-ion batteries (LIBs), which are the most widely used battery in the market, are the focus of various research and development efforts, from materials to systems, that seek to improve their performance. The separator is one of the core materials in LIBs and is a significant factor in the lifespan of high-performance batteries. To improve the performance of present LIBs, electrochemical testing and related surface analyses of the separator is essential. In this paper, we prepared a ceramic (Boehmite, γ-AlOOH) coated polypropylene separator and a porous polyimide separator to compare their electrochemical properties with a commercialized polypropylene (PP) separator. The prepared separators were assembled into nickel-manganese-cobalt (NMC) cathode half-cell and full-cell lithium-ion batteries. Their cycling performances were evaluated using differential capacity and electrochemical impedance spectroscopy with ethylene carbonate:dimethylcarbonate (EC:DMC) electrolyte. The ceramic coated polypropylene separator exhibited the best cycle performance at a high 5 C rate, with high ionic conductivity and less resistive solid electrolyte interphase. Also, it was confirmed that a separator solid electrolyte interface (SSEI) layer formed on the separator with cycle repetition, and it was also confirmed that this phenomenon determined the cycle life of the battery depending on the electrolyte. (Received July 13, 2021; Accepted August 20, 2021)