Crystalline silica is one of the mineral components in the earth’s crust. It has been used to make products engineering materials and used for applications such as potential electrode materials in the rechargeable battery and optoelectronic and sensing devices. One of the polymorphism of crystalline silica is alpha-quartz. Their typical structure shows spherical nanoparticles exhibiting the crystal habit of alphaquartz. However, synthesizing novel crystalline silica possessing thin two-dimensional layered nanostructure has not yet been reported. We report synthetic methods of preparing crystalline silica and its properties such as morphology, surface and crystallinity. The experimental evidence suggest that they are highly crystalline exhibiting the crystal structure of alpha-quartz such as XRD, SEM, TEM, XPS, and AFM. Our results on the complete characterization with the its properties and application for microalgae harvesting process will be discussed in the presentation.