A single spray plume is the basic unit of the entire spray plume and is an important factor in understanding the spray characteristics. However, since the multi-hole GDI injector has a narrow spray angle, the superposition of the spray plumes occurs severely. Therefore, the spray uniformity and the spray atomization characteristics of a single spray plume were analyzed in this study using a single-hole GDI injector. Five single-hole GDI injectors with different nozzle hole diameters were used in the experiment. The uniformity of the spray was evaluated through the analysis of the spray pattern images. In addition, the atomization characteristics were compared using the diameter distribution of the spray droplets obtained using PDPA. As a result, the larger diameter of the nozzle hole, the less uniformity of the spray, and the injection pressure did not have a significant effect on the spray uniformity. It is judged that the surface roughness of the injector has a greater effect on spray uniformity than the diameter of the nozzle hole. Also, the size of the spray droplets increased sharply when the diameter of the nozzle hole was 230 μm.