Background: Recent research has indicated that Particulate matter (PM) induced inflammatory response in the skin. And it has been reported that Ilantide, interleukin-1 receptor antagonist, and palmitoyl tetrapeptide-7(PT-7) have anti-inflammatory effects. Objectives: We asked whether PM10 can stimulate the human skin keratinocytes and fibroblast to induction of inflammation and by what mechanism play the changes. Also, we asked whether Ilantide and PT-7 can suppress PM10-induced inflammation. Methods: Human skin keratinocytes, HaCaT, and human skin fibroblasts, HS68, were used. The MTT assay was used to measure the cell viability when PM10 was treated and when Ilantide or PT-7 were added to the PM10-treated cells. Western blot was used to measure the protein expression of inflammatory cytokines induced by PM10, Ilantide, and PT-7. Results: We found that PM10 decrease the cell viability of HaCaT and HS68, and induce various inflammatory cytokines from these cells. Also, we found that Ilantide and PT-7 induce the various expression rate of different inflammatory cytokines in these cells. Although Ilantide didn’t change cell viability, PT-7 increased cell viability in both HaCaT and HS68. Conclusion: These data demonstrate that PM10 decreases cell viability by inducing the increase of inflammatory cytokines in these cells and PT-7 can reduce inflammatory response induced by PM10. Therefore, it is expected that a method using this new peptide can be devised to reduce skin damage caused by PM10.