Background: Excess sebum production contributes to the development of several skin diseases including acne vulgaris. Argon and nitrogen plasma pulses have been shown to induce coagulation of sebaceous glands in rat skin. Objectives: To evaluate the effect of argon and nitrogen plasma pulses on sebum production in oily human skin in vivo and in human sebocytes in vitro. Methods: Fifteen healthy individuals (aged 19-40 years) who were determined to have oily skin by Sebumeter measurement were treated with argon and nitrogen plasma pulses 3 sessions in a 1-week interval. UV images and objective measurements of sebum level and sebum excretion were performed at baseline and 2, 4, 8 weeks post-treatment. In two volunteered individuals, 2 mm skin biopsies were performed before and after treatments. Effects of argon and nitrogen plasma pulses on lipogenesis and proliferation of human SZ95 sebaceous were evaluated. Results: Argon and nitrogen plasma pulses induced a significant reduction of casual sebum level (29% at forehead and 53% at zygoma) and sebum excretion rate (25% at forehead and 40% at zygoma) at week 8. Biopsy samples revealed the reduction of Ki67 in basal sebaceous gland cell and epithelial duct at week 2. Adverse effects were minimal. Argon and nitrogen plasma pulses suppressed testosterone and linoleic acid treatment-induced lipogenesis and proliferative ability in cultured sebocytes. Conclusion: Argon and nitrogen plasma pulses can suppress sebaceous lipogenesis in vivo and in vitro.