The purpose of this study was to investigate effects of static stretching on flexibility, jump performance, and tensiomyography. Nine healthy adult men participated in this study. The participants were subjected to static stretching and cycling exercise treatment at 1 week intervals, respectively. The participants measured, anthropometric and body composition, and count movement jump and tensiomyography in biceps femoris after each treatment. As a results, the sitting trunk flexion showed significantly higher static stretching treatment compared to cycle exercise treatment, There was no difference in count movement jump between stretching and exercise. As for the contraction time of tensiomyography, static stretching treatment was significantly faster than cycle exercise, and sustain time showed significantly higher static stretching treatment compared to cycle exercise treatment, In conclusion, static stretching for a short period of time does not induce a decrease in Jump performance and can improve muscle contraction response time, flexibility, and range of motion in joint.