We estimated the spatio-temporally distributed soil moisture using Sentinel-1A/B SAR (Synthetic Aperture Radar) sensor images and soil moisture data assimilation technique in South Korea. Soil moisture data assimilation technique can extract the hydraulic parameters of soils using observed soil moisture and GA (Genetic Algorithm). The SWAP (Soil Water Atmosphere Plant) model associated with a soil moisture assimilation technique simulates the soil moisture using the soil hydraulic parameters and meteorological data as input data. The soil moisture based on Sentinel-1A/B was validated and evaluated using the pearson correlation and RMSE (Root Mean Square Error) analysis between estimated soil moisture and TDR soil moisture. The soil moisture data assimilation technique derived the soil hydraulic parameters using Sentinel-1A/B based soil moisture images, ASOS (Automated Synoptic Observing System) weather data and TRMM (Tropical Rainfall Measuring Mission)/GPM (Global Precipitation Measurement) rainfall data. The derived soil hydrological parameters as the input data to SWAP were used to simulate the daily soil moisture values at the spatial domain from 2001 to 2018 using the TRMM/GPM satellite rainfall data. Overall, the simulated soil moisture estimates matched well with the TDR measurements and Sentinel-1A/B based soil moisture under various land surface conditions (bare soil, crop, forest, and urban).