Interspecific hybrids of eggplants (Salanum species) are of prime concern to breeders, as they allow introgression of useful genes from wild species to cultivated ones. However, successful hybridization between species is usually very low. This hampers development of improved hybrids. In-vitro culture techniques may be used to overcome interspecific barriers. Seeds and excised embryos obtained from two crosses (Solanum (S.) melongena × S. torvum and S. melongena × S. anguivi) were inoculated on Murashige and Skoog (MS) basal medium supplemented with varying concentrations of 3-indoleacetic acid (IAA) and 6-benzylaminopurine (BAP). Addition of hormones in the culture medium significantly (P ＜ 0.05) improved root and shoot emergence, seed germination and seedling survival. However, excised embryos cultured on the same medium did not develop. Hybrid seeds cultured on MS medium supplemented with different concentrations of IAA and BAP germinated within 6-14 days (SM001-07 × ST004-03) and 4-10 days (SM001-07 × San005-01). Optimal concentration of IAA and BAP for seed germination and seedling survival depended on hybrid type. Regenerated plantlets were successfully transferred to the field after initial acclimatization. High levels of heterosis observed for number of seeds/fruit and fruit breadth suggested an opportunity for developing F1 hybrids with desirable fruit characters for improved fruit yield.