Eel Anguilla japonica is an economically important inland culture fish species in East Asian countries, particularly in Korea, Japan, China, and Taiwan. In this study, the effects of feeding frequency on larval growth and survival rate were investigated. Eel larvae (average length, 6 mm) were fed a slurry-type diet containing shark eggs three, five, seven, or nine times per day. Survival rates differed significantly among the four experimental groups. Feeding frequencies of at least five and seven times a day were required until 15 and 30 days after hatching, respectively, to maintain an acceptable survival rate. Leptocephalus growth improved with increased feeding frequency until 15 days after hatching. The results suggest that providing an opportunity for early food intake enhances eel survival and growth in the early leptocephalus stage.