Stored glycogen is important energy source during moderate to high intensity exercise. Therefore, many researches have performed to storing more glycogen in the body called `glycogen loading`(GL). However, GL has some problems that increase body weight, spend long-term, and so on. In the present study, (-)-hydroxycitrate (HCA) was used as the supplement. HCA is not interfere fat oxidation during moderate intensity exercise and reduce fat accumulation and possibly increases glycogen content in the body. Therefore, 300㎎ of HCA was mixed in the diet (P:23%; F:22%; C:55%) in experimental group (n=16, HCA) or without HCA (n=16, CON). Both groups were divided into two sub-groups, for which endurance exercise time analysis (n=8 in each group). The Sprague-Dawley male rats that have exercise trained and the diet supplemented for 3-week. Fat weight, blood glucose, lactate, Liver and muscle glycogen were analyzed. Fat weight and blood lactate concentration were significantly decreased in the HCA (p<.05). Glycogen contents were significantly increased after glycogen loading session (p<.05). Endurance exercise time was significantly lengthened in the HCA (p<.05). These results suggested that HCA ingestion before endurance exercise was meaningful to storing glycogen content in the body.