운동강도에 따라서 지질 과산화물의 생성과 항산화 효소의 활성정도를 파악하여 산화적 스트레스를 야기하는 운동강도가 있음을 규명하고 아울러 산화적 손상을 야기하는 강도의 운동을 실시할 때 항산화제 섭취를 통해서 산화적 손상을 방어할 수 있는지를 규명하고자 하였다. 이를 위해 62명의 피험자를 대상으로 항산화제와 운동처치를 5주간 실시한 후 비운동시(Non-exercise), 저강도(AT80%), 고강도(AT110%) 운동시 TBARS 생성 정도와 SOD, GPX, GRD, GSH, GSSG 활성화 정도를 비교 분석하고, 항산화제(위약처치집단, 복합비타민 섭취 집단, 타우린섭취 집단)에 따른 TBARS 생성정도와 SOD, GPX, GRD, GSH, GSSG 활성화 정도를 비교 분석하였다. 본 연구결과 적절한 강도를 벗어난 고강도 운동이 산화적 스트레스를 유발하며, 과도한 스트레스는 체내 항산화 방어 체계를 무너뜨리고 결국 산화적 손상의 원인이 되는 지질과산화물의 생성을 증가시키는 것을 알 수 있었다. 산화적 스트레스를 야기하는 운동강도는 AT(anaerobic threshold)수준 이상에서 존재함을 알 수 있었으며, 또한 타우린도 비타민 E나 C와 같이 항산화 기능이 있음을 알 수 있었다. 비타민과 타우린 섭취를 통해서 지질 과산화물의 생성 감소와 항산화효소의 활성을 증가시키는 것으로 보아 항산화제(비타민 E+C, 타우린)가 적정강도를 벗어난 과도한 운동에 의한 산화적 손상을 방어할 수 있음을 알 수 있었다.

The purpose of this study was to investigate protective effect of antioxidants supplementation and exercise intensity on the activities of lipid peroxidation. Sixty two middle school students(M, 14.7±0.5yr) were participated in the maximal exorcise test before and after Sweets antioxidants supplementation. The subjects were divided into three groups; vitamin C+E supplementation group(N=20), Taurine supplementation group(N=21), placebo group(N=21), Then each group was divided into three subgroup ; Non-exercise group, AT80%(low intensity) exercise group, AT110%(high intensity) exercise group. Maximal exercise testing( A strand protocol; 0.5kp/2min) by cycle ergometer was used as a test protocol. The programs of exercise groups were aerobic and fitness exercise for 5 weeks. Low intensity(AT 80%) group exercised for 60min per day(5days/week) and high intensity(AT 110%) group exercised for 120min. Vitamin C(2,000㎎/day), Vitamin E(1000IU/day), Taurine(1500㎎/day) were given for 5 weeks, Venous blood samples were drawn at the forearm antecubital vein at the immediately after exercise. The concentration of TBARS and the activities SOD, GPX, GRD, GSH, GSSG were measured in the blood to indicate the oxidative stress and antioxidative status. After five weeks of physical training, there were significant changes in indicators’ values only in high intensity exercise group compared with non exercise group and low intensity group and show high intensity exercise induced oxidative stress. The effect of vitamin C+E, taurine supplementation were beneficial to reduce the concentration of TBARS caused by high intensity exercise. Vitamin C and vitamin E seemed to have a synergistic effect each other. In conclusion, It has been found that high intensity exercise-induced oxidative stress caused lipid peroxidation (TBARS) increase. But dietary antioxidants had detoxifying effects on peroxides produced by high intensity exercise. Therefore, it has been suggested that vitamin C+E, taurine supplementation treatment for 5 weeks may protect oxidative damage from various oxidative stress produced by strenuous exercise.