Objective: Fetal development is a crucial time for metabolic programming, and poor nutrition during pregnancy could lead to obese offspring through effects on fetal programming. Particularly, the liver is critical role in the regulation of glucose and lipid metabolism. Thus, we assessed expression transcriptional factors regulating hepatic glucose and lipid metabolism in male offspring to investigate the effect of maternal food restriction during pregnancy. Method: Sprgue-Dawley rat were divided into three groups: 1) AdLib/AdLib (fed a normal diet during the entire experimental period), 2) FR/AdLib (given a 50% food restriction diet during pregnancy and a normal diet during lactation), 3) HF/HF (given a 45% high fat diet during experimental period). In 3-month-old male offspring, plasma metabolic profiles were measured by enzymatic colorimetric method. In the liver, lipid droplets were observed by staining with hematoxylin and eosin (H&E) and electron microscope and hepatic triglyceride levels were measured by enzymatic colorimetric method. The mRNA and Protein levels of hepatic glucose and lipid metabolism-related genes were determined by real-time PCR and western blot. Result: In 3-month old male offspring, while body weight was heavier in the HF/HF group, weight gain was significantly increased in FR/AdLib and HF/HF groups than control (P<0.05). Plasma glucose levels were significantly higher in the FR/AdLib and HF/HF (P<0.05). Moreover, hepatic triglyceride levels and the lipid droplets size in the FR/AdLib and HF/HF was significantly higher, and was much larger than control, respectively (P<0.05). The expression levels of phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) regulating hepatic glucose metabolism, were altered in FR/AdLib and HF/HF compared with control (P<0.05). Additionally, the expression levels of transcriptional factors regulating hepatic lipid metabolism SREBP1 and ACCα were significantly increased in FR/AdLib and HF/HF, while the expression levels of HNF4α were significantly decreased (P<0.05). Conclusion: Our findings suggest that maternal food restriction during pregnancy may increase the risk of non-alcoholic hepatic disease through the accumulation of hepatic lipid droplets and the alteration of hepatic glucose and lipid metabolism in later life of male offspring.