Objective Diabetes-specific quality-of-life instruments were originally developed as paper-and-pencil questionnaires. Current advances in technology have enabled the development of a computer-based questionnaire that provides various advantages over the paper-based mode of administration, such as automatic data entry, storage, and calculations. However, before implementing a computer-based questionnaire, its equivalence with the original paper-based questionnaire must first be demonstrated. This study was to test the measurement equivalence of the touch-screen computerized Diabetes-Specific Quality-of-Life questionnaire with its original paper-based counterpart. The times taken to complete the two modes of questionnaire, the user preference, and the ease of use were also explored. Methods The study used a randomized crossover design. Participants were 208 adults diagnosed with diabetes from two university hospitals. Use of a personal computer almost every day was reported by 39.9% of the participants, and 83.2% had ever used an automated teller machine. Participants completed the paper and computer modes of the Diabetes-Specific Quality-of-Life questionnaire and were then questioned (on paper) regarding their preference and the ease of use of the modes. The measurement equivalence was evaluated using quadratic weighted kappa coefficient, ICC, and Cronbach’s alpha comparisons. Results The quadratic weighted kappa coefficient ranged from 0.703 to 0.823, and the ICCs ranged from 0.95 to 0.92 for the total scale and subscales. Cronbach’s alpha values did not significantly differ between the two modes of questionnaire presentation. Among the participants, 82.7% preferred to use the computerized questionnaire, and 86.9% responded that this questionnaire was easy to use. There was no difference in the time taken to complete the two questionnaires. Conclusion The touch-screen computerized Diabetes-Specific Quality-of-Life questionnaire was equivalent to its original paper-based counterpart. Participants preferred the computerized questionnaire over the paper-based questionnaire and reported that it was easy to use. The computerized Diabetes-Specific Quality-of-Life questionnaire may thus be feasible for use in clinical practice as an alternative to the original paper-based tool.