Objective To examine the changes of white adipose tissue and lipogenesis-related genes expression induced by radiation exposure Methods C57BL/6 mice were exposed to 137 Cs γ-rays at a single dose (5 Gy) or fractionated doses (1 Gy × 5 times, 0.5 Gy × 10 times, or 0.2 Gy × 25 times) at the age of 2 months. After 6 months later, white adipose tissue was isolated from mouse. 2 and 25-month-old mice were studied as the young and old references. The real-time RT PCR was performed to examine the mRNA expressions of genes related lipid metabolism. Results The weight of white adipose tissue in the irradiated group presented had tendencies to increase compared to non-irradiated group. The patterns of SREBP-1c, ACC, FAS, ACL, GLUT4, ME1 and G6PD2 mRNA levels were relatively lower in γ-irradiation groups than in non-irradiation group. The mRNA levels of leptin and DGAT are relatively higher than non-irradiation group. Conclusion The physical agent such as γ-ray could triggers the biological response resulting in the fat accumulation of white adipose tissue in mice.