Objective: To evaluate the effect of tomato peel extract on fasting blood glucose and HOMA-IR in high fat fed/streptozotocin-induced type 2 diabetic rats. Methods: Twenty male Wistar rats were randomly divided into five groups, as negative control/normal group, positive control/diabetic rats without treatment, and 3 groups of diabetic rats treated with a dose level of 50 mg/kg b.w., 100 mg/kg b.w., and 150 mg/kg b.w. tomato peel extracts, for 4 weeks. Type 2 diabetes mellitus was induced by high-fat diet for 5 weeks and a low dose of streptozotocin (30 mg/kg, i.p). At the end of treatment, fasting blood sugar level, fasting/basal insulin, and homeostasis model assessment of insulin resistance (HOMA-IR), were measured. Fasting blood sugar levels were measured before and post-treatment. Results: There is significant decrease in fasting blood glucose level in a dose of 50 mg/kg b.w. tomato peel extract treatment. Tomato peel extract significantly reduces fasting serum insulin level and HOMA-IR. Conclusion: Tomato peel extract improves insulin resistance in type 2 diabetes caused by high-fat diet and low dose streptozotocin in male rats.