Objective： It is well known that defective insulin secretion and hyperglucagonemia are important pathophysiologies in patients with type 2 diabetes. But mechanisms for glucagon dysregalation of pancreatic alpha cell remain unclear. We tried to determine the effect of lipotoxicity on viability, apoptosis, glucagon secretion, using alpha TCI cell Methods： Alpha TCI Cells were exposed to 5 mM or 25 mM Glucose with or without 0.25-1.0mM palmiate for 72hr. Cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay. Secreted glucagon protein in the media was measured using Glucagon ELISA kit. We also measured apotosis marker. Results : The high concentrations of palmitate caused a significant reduction in alpha TC cell viability and cell viability decreased as the palmitate concentration increased. Cell apoptosis also decreased with increasing palmitate concentration. Exposure of alpha TC cell to palmitate significantly increased glucagon secretion and decreased content Conclusion： In conclusion, lipotoxicity on pancreatic alpha cells may reduce cell viability but increase glucagon secretion and contribute to hyperglycemia in type 2 diabetes.