Objective: Diabetic people are at significantly higher risk for many forms of cancer by epidemiologic studies. Colorectal cancer also shows higher prevalence in diabetic patients. Many inflammatory cytokines and IGF-1 signal are associated with higher prevalence of colorectal cancer. However, there is not sufficient data of cancer prognosis and diabetes. Recently, Akt signal is associated with chemoresistance and colorectal cancer prognosis. In this study, we aimed to validate the relation of hyperinsulinemia and Akt isoforms, furthermore investigate the chemoresistance by Akt isoforms. Methods： Immunohistochemistiy stains of Aktl and Akt2 were analyzed in 10 patients with colorectal patients with diabetes (n = 5) and non-diabetic patients (n = 5). Colorectal cancer cell line, HCT116 is used for analysis of oxaliplatin sensitivity assay by insulin concentration luM, 2uM. Aktl and Akt2 expressions are compared by western blot. Knockdown of Aktl and Akt2 is performed by Lipofectamine RNAiMAX. Results: AKT1 expression is highly expressed in colon cancer tissue of diabetic patients. Aktl, rather than Akt2 expression is highly increased by insulin in colorectal cancer cell line. Knockdown of Aktl sensitize the colorectal cancer cell line to chemotherapy. Conclusion： Aktl expression is increased in diabetic colorectal cancer patients, and is associated with chemoresistance of oxaliplatin.