Objective : Uncoupling protein 2 gene variant has been associated with obesity with G allele is associated with a greater risk of weight gain. Intervention should be addressed to develop treatment for those with GG genotype of UCP2 gene. This study was aimed to analyze the impact of several dietary regimes on body weight and body composition between UCP2 gene variants. Methods : Subjects were 76 male and female adults with overweight or obesity status. Changes in body weight and body composition after 4 weeks of following dietary regime： intermittent fasting diet (IFD), low calorie diet (LCD) and low calorie diet with exercise (LCDex) were analyzed in this study. The -866 G/A UCP2 gene variation was analyzed and subjects were separated into 2 genotype groups : GG and AA+GA. Results : In this study, we showed that in all 3 dietary regime, subjects reduced their total calorie intake and no differences were observed (GG pANOVA = 0.499 and AA + GA pANOVA = 01.29). For AA+GA genotypes, subject in LCDex (△weight = -2.0 土 0.4 kg) group had a greater weight loss than those in IFD (△weight = -1.1 土 0.2 kg; p = 0.025) and LCD (△weight = -1.0 ± 1.0 kg; = 0.144) groups. By contrast, for GG genotype, subject in LCDex (△weight = -1.5 土 0.6 kg) group had a similar weight reduction compared to those in IFD (△weight = -1.6 ± 0.2 kg; p = 0.389) group but higher than LCD (△weight= -0.4 ± 0.4 kg; p = 0.094) group. Conclusion : This study revealed that intermittent fasting diet has a similar beneficial effect in a weight loss as in low calorie diet with exercise for those with GG genotype. This study can be used a basis for a development of personalized weight loss program based on UCP2 gene variants.