Objective: General objective: To study the association of dyslipidemia in patients with cholelithiasis Specific objectives: To investigate the level of cholesterol in patients with cholelithiasis. To investigate the level of triglycerides in patients with cholelithiasis. To investigate the level of HDL cholesterol in patients with cholelithiasis. To investigate the level of LDL cholesterol in patients with cholelithiasis To study the age and sex distribution in cases of cholelithiasis. To study the correlations between lipid profile parameters Methods: A prospective study was designed to find out lipid profile in the sera of patients with cholelithiasis in comparison with normal individuals (control) in the surgical ward of Tribhuvan University Teaching Hospital .50 (male = 17, female = 33) symptomatic gallstone patients (aged 45.92 ± 13.41 years), and 50 (male = 27 and female = 23) apparently healthy controls (aged 46.8 ± 11.8 years) were included in the study. Lipid profile was estimated and compared between cases and control as well as among different demographic characteristics of the population included in the study. Different groups were compared by using Student’s t test and p < 0.05 was considered statistically significant. Results: This study found a significant increase (P < 0.05) in serum: TG, in patients with cholelithiasis compared to the apparently healthy controls. The study also showed that there was a significant decrease (P < 0.05) in serum HDL-c in gallstone patients compared to control. However there was no significant correlation found between the level of TC and LDL in patients with cholelithiasis compared to control. Conclusion: Our study clearly showed the association between dyslipidemia (hypertriglyceredemia and low HDL level) with cholelithiasis which can be the cause or effect of gall stone formation.