Objective: This study aimed to determine the effect of Moringa seeds extract against blood gluocose level and pancreatic islet area in metabolic syndrome rats. Methods: This study is laboratory experimental research using pretest-postest control group design for blood glucose measurement and postest only control group design for pancreatic islet area. Male Wistar rats were divided into four groups randomly. Group I were fed with standard pellet, group II were fed with high fat high fructose diet only, group III were fed with high fat high fructose diet and Moringa seeds extract at dose of 150 mg/kgBW, and group IV were fed with high fat high fructose diet and Moringa seeds extract at dose of 200 mg/kgBW. The metabolic syndrome is induced with high fat high fructose diet for 53 days which consist of duck yolk 2 mL/200 gramBW, beef tallow 2 ml/200gramBW, fructose 0,36 g/200 gramBW, and oxidized oil 1 mL/200 gBW. Moringa seeds extract were given for 28 days. Blood glucose level was analyed with repeated ANOVA test. Pancreatic islet area was analyzed with one-way ANOVA and post hoc Tukey HSD test. Results: There were significant difference based on repeated ANOVA between the blood glucose level before-after given with high fat high fructose diet, and after given with Moringa seeds extract (p < 0.05). One-way ANOVA test for pancreatic islet area showed that there were difference between 4 groups (p < 0.05). Tukey HSD post hoc test showed significant difference between group I with group II and group I with group III. Conclusion: Moringa oleifera seeds extract with the dosage 150 mg/kgBW can lower blood glucose significantly but does not lower pancreatic islet area on significant level. Moringa oleifera seeds extract with the dosage 200 mg/kgBW can both lower blood glucose and mean islet area on significant level.