Objective: Imbalance in production of oxidant and antioxidant may inflict wound healing deficiency which may result in diabetic complications. Piper sarmentosum crude extracts consist of phenolic compounds that are major antioxidants to protect cultured skin cells against oxidative damage while, hydrogen peroxide (H2O2) is widely known to be toxic to cells and excessive production of H2O2 may cause oxidative stress in cell. However, low dosage of H2O2 also needed in wound healing process and less study has been done to investigate the important of oxidant and antioxidant produced in our body. An experiment on fibroblast cell was conducted using P. sarmentosum and low dosage of H2O2 to observe and compare the roles of each treatment in wound healing activity. Methods: DPPH assays were done to investigate the ability of P. sarmentosum and H2O2 in scavenging DPPH free radical while AlamarBlueTM cell viability and scratch assay were done to observe cell proliferation and cell migration after treated with P. sarmentosum and low dosage of H2O2. Results: P. sarmentosum shows a great ability of scavenging free radical while H2O2 shows to be otherwise. However, cell proliferation and migration of cell after treatment exhibit positive cell viability and migration not only for P. sarmentosum but also for low dosage of H2O2 treatment. Conclusion: This suggests that oxidants such as H2O2 not only give harm to the cell but also has it on role in promoting cell proliferation and migration in low dosage.