The purpose of this study was to investigate the effect of vitamin B-6 administration for 6 weeks on changes of blood glucose and lactate after Taylor test protocol exercise on treadmill. All subjects were 16 athletes males, who were divided into 2 groups: Experimental(8) and Control group(8). Blood sampling were collected 4 times : rest, immediately exercise, post-15, 30min after exercise. Data analysis were tested by SPSS(2-way ANOVA) within and between groups. The result was following as : 1. In the comparison of blood glucose within `groups, experimental group was showed significantly increase of blood glucose at immediately exercise after vitamin B₆ administration, but both groups in other condition were showed no change of blood glucose. 2. In the comparison of blood glucose between groups, in experimental groups were significantly higher than control group at immediately exercise after vitamin B₆ treatment. In other condition all groups were showed no change of blood glucose. 3. In the comparison of blood lactate within groups, experimental group was showed significantly decrease at recovery 30 min exercise after vitamin B_6 administration, but both Groups were showed no change of blood lactate in other condition. 4. In the comparison of blood lactate between group, blood lactate in experimental groups were significantly decrease in comparison with control group at post-30 min. exercise after vitamin B₆ treatment. So, vitamin B₆ administration can be effected to facilitate for the efficacy for energy metabolism, and to decrease blood lactate at recovery stage. These effects should be suggest as result that vitamin B₆ can be activited to metabolism for exercise.