Objective: Hyperlipidemia is one of diabetes mellitus type 2 complication that can increase the level of ureum and creatinine in the blood. Creatinine is produced in the muscle by the nonenzymatic conversion of creatin and phosphocreatine and is constant proportionally with muscle mass. Meanwhile, ureum is produced as an end product of protein, in several studies increase in ureum was associated with the complication of incident diabetes. This study is aimed to know the association of the increase of ureum and creatinine levels in hyperlipidemia condition induced by high cholesterol diet using quail egg yolks. Methods: This research used post-test only control group design. The rats divided into two groups, A group and B group. Both groups were fed ad libitum while B group were only given quail egg yolk each day for four weeks with a dose of 5 ml/200 grams of body weight. All rats terminated to taken the blood serum to measure the levels of ureum and creatinine. All data were statistically analyzed using software application type independent sample T-test. Values were considered significant at p < 0.05. Results: Mean of creatinine (mg/dL) levels were 11.06 ± 0.59 (A), 46.47 ± 1.60 (B). Mean of ureum (mg/dL) levels were 0.63 ± 0.38 (A), 3.2 ± 0.11(B). The results showed significant differences in ureum and creatinine levels between all groups after the intervention of quail egg yolks in male Wistar rats with the P-value = 0.000 and P-value = 0.000 (p < 0.05). Conclusion: High cholesterol diet using quail egg yolks significantly increase the ureum and creatinine levels in blood serum. This research strengthens the previous research that diabetes could lead to elevation of ureum and creatinine caused by hyperlipidemia.