Objective: A high cholesterol diet can cause hyperlipidemia condition and it is one of the risk factors for Diabetes Mellitus (DM). The quail egg yolks have the highest cholesterol levels compared to other egg yolks. Hyperlipidemia has a bad effect that can decrease the Superoxide Dismutase (SOD) inhibition rate and increase the levels of Reactive Oxygen Species (ROS). SOD is nonenzymic antioxidants play an important role in reducing tissue damage due to free radical. Decreased SOD inhibition rate in hyperlipidemia causes a decrease in kidney protection ability against oxidative stress due to increased levels of ROS. This condition can cause kidney failure or is called diabetic nephropathy which is a complication of diabetes mellitus. The aim of this research is to know the effect of quail egg yolk on the SOD inhibition rate of kidney tissue in the hyperlipidemia rats model. Methods: This research used a post-test only control group design. The rats divided into two groups, A group and B group. A and B group were fed ad libitum while B group was given well quail egg yolk with a dose of 5ml/ 200g of body weight given each day for four weeks. All rats terminated to taken the kidney organ to measure the inhibition rate of SOD, all data were statistically analyzed using a software application type independent sample T-test. Values were considered significant at p<0,05. Results: Mean of SOD inhibition rate (%) were 67.81 ± 2.11 (A), 22.79 ± 1.78 (B). The results showed a significant difference in the SOD inhibition rate between all groups after the hyperlipidemia intervention of quail egg yolks in the male rats P-value = 0.000 (p < 0.05). Conclusion: The intervention of quail egg yolks significantly decreases the SOD inhibition rate in kidney tissue of the male rats.