Objective: Obesity is characterized by chronic low-grade inflammation. NLRP3 inflammasome is part of innate immune system which senses the danger signals and causes insulin resistance. Activation of inflammasome complex leads to cleaved caspase-1 which further activates the IL-1β and IL-18 to cause inflammation. To date, the role of inflammasome in obesity-related metabolic diseases has been focused mostly in immune cells, whereas its role in adipocytes is relatively unknown. The aim of this study is to examine the expression profile of the inflammasome-related genes in adipocytes and stromal vascular fraction (SVF) isolated from high fat diet-fed (HFD) mice. Methods: Adipocytes and SVF were isolated from control mice and mice fed high fat diet for 11 weeks. The expressions of inflammasome-related genes were measured using RT2 profiler PCR array. Further analysis was performed to obtain data on pattern of upregulated and downregulated genes by HFD from adipocytes and SVF, respectively. Results were validated by real-time PCR. Results: Total of 84 genes were measured, out of which 28 genes were upregulated including bcl2, caspase-8 and 12, nod1, pea15a, while irf4 was the only downregulated gene in adipocytes. Comparison with results from SVF showed common and distinct gene regulation by HFD. Real-time PCR analysis further confirmed that the upregulation of NLRP3 and caspase-1 in HFD adipocytes were comparable to SVF. Conclusion: These findings suggest that NLRP3 inflammasome complex is activated in not only immune cells but also in adipocytes in obesity, which may be responsible for development of inflammation and metabolic disturbance in type 2 diabetes.