Objective Hyperglycemia considered as a condition with intracellular myo-inositol deficiency. Restoration of myo-inositol to normal level might be beneficial for the prevention and treatment of the complications associated with diabetes mellitus (DM). However, little information is available concerning the myo-inositol content in Korean fruits. Therefore, we analyzed the contents of myo-inositol in Korean fruits, major useful sources of myo-inositol. Methods Frequently consumed 6 fruits (apple, water melon, tangerine, oriental melon, green and gold kiwifruits) were analyzed for their sugar contents by high-performance anion-exchange chromatography (HPAEC, Dinonex model DX-600) and electrochemical detection (ED) coupled with CarboPac MA-1 column. Myo-inositol, glucose, fructose, and sucrose in the fruits were successfully separated within 25 min using 0.612 M NaOH eluent at a flow-rate of 0.4 mL/min. Glycemic index (GI) and insulin index from 15 healthy subjects were also analyzed. Results Contents of myo-inositol in tangerine, green kiwifruit, and water melon were 320±27, 190±0.04, and 63±3 mg/100g, respectively. However, myo-inositol was not found in apple, oriental melon, and gold kiwifruit. The GIs of tangerine, green kiwi, water melon, oriental melon, and gold kiwi were 54.8±6.3, 42.7±5.1, 53.5±5.0, 51.2±5.0, 45.8±5.9, respectively. Insulin index of each fruits were 60.2±10.6, 54.8±12.9, 83.7±13.5, 147.7±30.0, 59.8±12.0, respectively. Conclusion Fruits are high in fiber, vitamin, water and sugars, also contain various phytochemicals. Thus, intakes of fruits were emphasized for health and disease prevention. However, many patients of DM restrict intakes of fruits because of glycemic control. More investigations about Korean fruits are needed for DM patients.