Objective Adipocyte fatty acid-binding protein (FABP4) and Tumor necrosis factor-alpha (TNF-α) are members of adipocytokines and plasma biomarkers closely associated with obesity and metabolic syndrome. We evaluated whether serum FABP4 and TNF-α are associated with renal function measured by estimated glomerular filtration rate (eGFR). Methods A total of 493 subjects (Men:women=325:168, 40.8±6.3 years) were selected from participants in a medical check-up program at Health Promotion center at 2003. Baseline serum FABP4 and TNF-α levels were measured, and glomerular filtration rate was calculated by MDRD (modification of diet in renal disease study) equation for renal function. The subjects were followed up 4 years later. Results Baseline levels of serum FABP4 and TNF-α were 9.95± 4.32ng/mL and 3.48±1.74pg/mL, respectively. Serum FABP4 level at baseline was shown to be negatively associated with eGFR (r=-0.293 p＜0.001 in 2003, r=-0.178 p＜0.001 in 2007), and also when adjusted for sex, age, BMI, Triglyceride, LDL-cholesterol, fasting blood sugar and systolic blood pressure (r=-0.251 p＜0.001 in 2003, ß=-0.209 p＜0.001 in 2007). On the other hand, baseline serum TNF-α level was shown to be negatively associated with eGFR only in 2003(r=-0.122 p=0.008 in 2003, r=-0.044 p=0.346 in 2007), and when adjusted for same clinical factors serum TNF-α didn't have significant relationship with eGFR. Both serum FABP4 and TNF-α were not significantly related to change in eGFR during 4 years. Conclusion It seems that serum FABP4 levels could be a related with renal function in healthy adults, although the mechanism needs to be elucidated.